FastQCFastQC Report
Thu 28 Apr 2016
ERR1041735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284575
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT502763.9138236381682656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296042.3045754432399823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231641.803242317498005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180201.4027985909736684No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA112530.8760095751513146No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85080.6623202226417297No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62450.48615300780413756No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60580.4715956639355429No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.4105638051495631No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC49770.3874433178288539No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA44950.34992118015686124No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA42700.33240565945935424No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG40690.3167584609695814No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38340.29846447268551857No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA35230.27425413074363114No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA33300.259229706323103No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA29700.23120487320709182No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28500.22186326216842145No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG27930.21742599692505304No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA27640.21516844092404103No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.20878500671428296No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA25950.2020123387112469No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.19944339567561256No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA25010.1946947433976218No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA24680.19212580036198743No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24250.1887783897397972No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA23900.1860537531868517No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA19960.15538213027655062No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA19600.1525796469649495No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19160.14915438958410368No Hit
GAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.14713037385905844No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA18740.14588482572056904No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18640.14510635813401318No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA18160.14136971371854504No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17540.13654321468189867No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17330.13490843275013137No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.13490843275013137No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA17120.13327365081836406No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA17060.13280657026643053No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA16500.12844715178171767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16440.1279800712297842No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.1276686841951618No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA15910.12385419302103809No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA15110.11762645232859117No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14910.11606951715547945No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA14610.11373411439581185No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA14170.11030885701496604No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC14020.10914115563513224No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA13560.10556020473697525No Hit
GAGTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAA13380.10415896308117471No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA12870.10018877838973979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA358.869965E-426.4285721
GTCGTCC400.001931309723.1250028
AAGACGG1155.456968E-1222.521745
GACTTTT7900.021.0759494
CGAACGT450.0038260520.5555554
GAGGACT8200.020.3048761
GGACTTT8400.020.0416663
AGGACTT8450.019.7041442
GCAAGAC1800.018.53
TTATCTA1002.8755858E-718.54
GCGCAAG1409.458745E-1118.51
ACGGACC1409.458745E-1118.58
GGTTATC1002.8755858E-718.52
TCGAACG500.007035321618.53
CGCAAGA1552.1827873E-1117.9032252
CGTTATA852.7235204E-517.4117642
GCGTTAT1401.869921E-917.1785721
GTATTAG1757.2759576E-1216.9142861
GACGGAC1452.9795046E-916.5862067
TTCGGGC1452.9795046E-916.58620635
Produced by FastQC (version 0.11.5)