FastQCFastQC Report
Thu 28 Apr 2016
ERR1041734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395238
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1239061.6754836017447987No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG927121.2536716194935174No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA794281.0740425122220543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT712580.9635660136969223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503900.6813844260319951No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC457910.6191957581351675No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG454000.6139085719756416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT406210.5492859053352982No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG368940.4988886091292802No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT366680.4958325884846438No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG364930.4934662008173368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT299910.40554475731545087No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA242600.3280489417649574No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT220530.2982054127264058No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA218250.29512234765128587No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC213040.2880772735103319No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC198360.26822666153543673No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC192260.2599781102379667No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT181790.24582035087985No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA170550.23062138094811824No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167280.22619961656406462No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG158640.21451642259518897No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT145910.19730264259243585No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC145000.1960721210054362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC144540.19545009910431552No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT143040.19342176681805237No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG130940.17705988637552977No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT124900.16889246836951022No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT120380.1627804270802373No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA120030.16230714954677591No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG117360.15869671807722752No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114140.15434256476938268No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT107880.14587765802804453No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG99570.13464069716214677No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG96230.13012427727140086No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT95150.1286638780252914No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94630.1279607228327202No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG94600.12792015618699493No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT93820.1268654233981381No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT88820.12010431577726098No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG86570.11706181734786629No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC85950.11622344000287753No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT83600.11304571942106528No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA81820.11063876510803304No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC77810.10521635679608958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1851.8189894E-1116.035
AAGACGG13100.015.1106875
TTAGAGT7500.015.0466664
TCGTTTA8200.014.89024430
TATACTG7450.014.6510075
CGTTATA2400.014.6458342
ATTAGAG8600.014.6279073
TATACCG2059.276846E-1114.4390245
GATACAT10150.014.3990141
AAAAGTA33400.014.3458082
TATAACG13000.014.2307692
TGTACCT48850.014.08802435
GGTTATC7750.013.8451622
CAAGACG14550.013.7319584
TAGACCG950.001246193913.6315795
TATCTAG11550.013.6147181
AGTACTC39500.013.5354435
TAGAGTG7800.013.5192315
CGAGCCG10550.013.5023715
ATGTACC50200.013.41434334
Produced by FastQC (version 0.11.5)