FastQCFastQC Report
Thu 28 Apr 2016
ERR1041733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5079088
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT770971.5179299905809862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478760.9426101693847398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT403810.7950443071669558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT245010.482389751860964No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA173110.3408289047167523No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC157380.3098587777963288No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132690.261247688561411No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA126660.2493754784323485No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG123510.2431735776186591No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109440.21547175398417984No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT99400.19570442567642066No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG94790.18662799305702127No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG89550.176311180274884No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT79730.15697700059538247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70910.13961167831705218No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG63060.124156147717858No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA59110.11637916098323163No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC57320.11285490623513512No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56150.1105513430757648No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA52760.1038769164857943No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA52530.10342407928352491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT3000.016.0333334
CCGCGTA700.002594130815.8571429
AAGACGG11750.015.5872345
TCGAACG3100.015.5161293
GACGGAC10350.015.371987
ACGGACC10650.015.1126768
CGAACGA4750.014.816
CAAGACG12600.014.5357144
CGTTATA1152.212676E-514.4782612
ACCGTCG6550.014.1221388
TCTACGC1601.7870661E-713.8753
CCGTCGT6600.013.7348489
CGCAAGA12400.013.7258062
AGACGGA12300.013.6869936
TCGTTTA6400.013.585937530
GCGCAAG12600.013.50793651
TACCGTC6600.013.4545457
GGTTATC6250.013.3199992
CGGACCA11050.013.2262459
CGAGCCG8350.013.071856515
Produced by FastQC (version 0.11.5)