FastQCFastQC Report
Thu 28 Apr 2016
ERR1041732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11864775
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1571191.3242476153150817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1041130.8774966234083664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT885190.7460655596081679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT678460.5718271100800478No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA403500.3400823024456848No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC343390.28941973193760523No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326860.27548773575562957No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG250770.21135672610732187No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA235870.1987985444308889No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228820.19285658598667063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204620.17246007614977948No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT203460.17148239220718473No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG176710.14893666335855504No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG157960.1331335823898894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT141270.11906673325031449No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA139240.11735578635077362No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA130280.10980402072521393No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA125230.10554772425098663No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA124320.10478074805464073No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC120440.1015105638328582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA3550.015.6338032
TACCGTC17350.015.5677227
TATACTG16250.014.6861535
ACCGTCG16600.014.5993988
CCGTCGT17050.014.2140769
ATACCGT21650.014.1847586
AAGACGG24700.014.1558715
CGAACGA7850.014.14012716
GACGGAC22800.014.0372817
CGTCGTA16950.013.86135810
ACGGACC23500.013.7765968
TTAGACT8800.013.6647734
TAGAGTG15100.013.2317875
TCGTTTA16800.013.21428730
CACTCTA21350.012.8243579
ACACGCT20300.012.7586219
TAGAGTC26100.012.758625
GAACAGT173150.012.5755131
TATACCG4300.012.4767445
GTATTAG29400.012.4591841
Produced by FastQC (version 0.11.5)