FastQCFastQC Report
Thu 28 Apr 2016
ERR1041731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005756
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT311971.0379085993673471No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234460.7800367029126782No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA168290.5598924197439845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165410.5503108036713559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC130290.4334683187856898No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG116260.3867912099318774No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG112000.37261840282444747No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG111790.3719197433191517No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT109980.3658979637735066No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG87790.29207294271391293No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA61370.20417492304764592No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56390.18760671192205888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54080.17992145736380466No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC52760.1755298833305165No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.16817732377478412No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC50240.16714596926696643No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC47420.1577639701958509No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46620.15510241017567628No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44310.14741715561742205No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA42450.14122902857051603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40660.1352737880253753No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT40510.13477474552159258No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG37450.12459427844442462No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT35820.11917134990331883No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35110.11680921538541386No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC33350.11095378334102968No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC32940.10958973383069018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT1900.018.54
CGTTATA902.1545711E-618.52
ATTAGAC2400.017.7291683
ACGGACC6600.017.659098
AAGACGG7200.017.4722215
GTCGTTA752.068666E-417.26666634
TCGAACG2050.017.1463413
TAGACTA1801.0913936E-1116.4444455
GACGGAC7150.016.30077
CGGACCA6850.016.204389
CGCAAGA8250.015.9212132
AAAAGTA15100.015.1920542
AGACGGA7800.015.1794876
TATACTG3300.015.1363645
CAAGACG9350.015.0374334
GCAAGAC11550.014.4155853
GCGAAAG8050.014.24844718
GTTATCT3900.014.230773
GCGCAAG8200.014.2134141
AAGTACT18900.013.99735454
Produced by FastQC (version 0.11.5)