FastQCFastQC Report
Thu 28 Apr 2016
ERR1041728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6475983
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT279910.4322278177691325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194120.2997537207864814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174120.2688703784429329No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA104800.16182871388019396No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC88820.1371529233476987No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG80900.12492311977965354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71160.10988293205834543No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT68120.10518866402212607No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG66870.10325845512565428No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG66150.10214665480128653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC13200.014.7159097
TCGTTTA7700.014.41558430
AAGACGG15550.014.2765285
AACCGTA2101.364242E-1014.0952387
ACGGACC13750.013.9927288
CGAACTA2950.013.7966124
TATACTG11800.013.796615
CGAACGT4050.013.7037044
CAAGACG16250.013.6615384
TAGAGTG10550.013.502375
TACACCG5300.013.264155
TTAGACT7650.013.0588244
TAGACTA5250.013.03809455
TCGAACG4300.012.9069783
TACGACG7750.012.8903225
GTACCGT1307.0075694E-512.8076936
ATACCGT13050.012.7586216
ACCGTCG9650.012.652858
AGACGGA16250.012.409236
TACCGTC10750.012.39069757
Produced by FastQC (version 0.11.5)