FastQCFastQC Report
Thu 28 Apr 2016
ERR1041727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002325
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT653552.17681296994829No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC535151.7824519330851922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481471.6036571656965852No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA375531.2507972987601277No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG368341.2268491918762958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT354021.1791528232286643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT258110.8597003988575521No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC233770.7786298951645808No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG211370.7040210503526434No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA201920.6725454439476073No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG201490.6711132205873781No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT193400.6441674369030668No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG181720.6052642535368423No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155810.5189644692030343No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA140950.46946949447511516No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC105950.3528931744564629No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104260.34726420357556226No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT101410.3377715603740435No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC95520.31815343109090455No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT94480.31468944901035034No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC89860.2993013747678882No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT87780.2923734106067798No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85480.284712681005554No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA83810.27915032516466404No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG80150.26695977284271355No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA74530.24824094659971854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT73890.2461092653193775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC73300.24414412163906307No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT73190.24377773891900442No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG72520.2415461350786474No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC70320.23421848067747497No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66630.22192800579550848No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT65040.21663211011466113No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA63560.21170259715387243No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC59630.19861274179177804No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG59550.1983462816317354No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.19061893699049903No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG56710.1888869459502219No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA55980.18645549698983288No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA55840.18598919170975828No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT54370.18109298626897488No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA53510.17822853954851656No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC53270.17742915906838866No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.17363210178778113No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA51800.17253295362760526No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA51370.1711007302673761No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT49050.16337338562613973No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG48960.16307361794609176No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48040.16000932610560148No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT47450.15804418242528706No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA46070.15344774466455163No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT45690.15218205890434913No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG45130.1503168377840507No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44270.14745239106359237No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG43830.1459868601833579No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT43030.14332225858293157No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42540.14169019010267042No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA42500.14155696002264911No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.1385925907421748No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA41520.13829282306212684No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG40310.13426261314148202No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT38580.12850041218056008No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA38500.12823395202051743No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37380.12450350977992056No Hit
GAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.1238706668998193No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.12120606529939298No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36380.12117275777938764No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT35630.11867469377898794No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA34010.11327887553812463No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT33600.11191326721790612No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33420.1113137318578102No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33250.11074750401771961No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT33110.11028119873764498No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA32930.10968166337754906No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA32030.10668398657706944No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA31960.10645083393703214No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT31540.10505191809680832No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA31380.10451899777672305No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA30860.10278700673644592No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG30770.10248723905639796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAC303.6008493E-430.8333348
TAAGTTC2450.024.1632673
CACGCAG5800.023.9224135
GATACAT4150.021.8433741
CGTTATA957.1559043E-921.4210532
TCGGTAG705.1064453E-621.14285928
ATAAGTT3000.020.9666672
ACCGGCT1151.3460522E-1020.91304424
TACGCAG1151.3460522E-1020.9130445
TTAACGG909.491123E-820.55555335
TCCGAAC555.1457493E-420.1818182
CTCGGTA759.273937E-619.73333427
GCGGAAT759.273937E-619.73333419
AACCGGC1254.129106E-1019.2423
GAGGACT15850.018.9085161
AAGTTCC3150.018.793654
GCGTTAT1700.018.5000021
GATACAA6900.018.51
CCGGCTT1409.458745E-1118.525
AAAAGTA18400.018.52
Produced by FastQC (version 0.11.5)