FastQCFastQC Report
Thu 28 Apr 2016
ERR1041726.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041726.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499482
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT995792.845535424957179No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC666691.905110527786684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635041.8146685709485004No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG603171.7235979496394038No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA543111.551972549080121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT493691.4107516483868183No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG359851.028295044809489No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG303940.8685285422242492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290890.8312373088359933No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG283970.8114629536599989No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT281260.80371895040466No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA258720.7393094177938335No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC227530.6501819412130138No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184460.5271065832028855No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT167050.47735636302744233No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA154010.4400937052969554No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC154010.4400937052969554No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137860.3939440180003783No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC136130.3890004292063797No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC135430.3870001331625652No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT135120.38611428777173307No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT121980.34856587346355833No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112810.3223619952895886No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG111650.3190472189884103No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC110030.3144179624298682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC108540.3101601894223202No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT107660.30764553153866775No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA101560.2902143802997129No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA100840.28815693294036093No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG100560.2873568145228351No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT96060.27449776852688484No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA88940.25415190019551465No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA84430.2412642785417956No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG83860.23963546604897526No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT82880.236835051587635No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG78640.22471897269367297No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT77100.22031832139728108No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG77100.22031832139728108No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA75110.2146317655012942No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT73370.20965960104952674No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT66420.1897995189002258No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA64770.18508453536837738No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64140.18328426892894434No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.18316996629786925No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA63840.18242699919588098No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC63610.1817697590671991No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA62730.1792551011835466No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA62710.17919794986800905No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT62320.1780834992150267No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60740.17356854528755972No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT59150.16902501570232395No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT58790.16799629202264793No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC58550.16731047623619724No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA58500.16716759794735336No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT58250.1664532065031339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG53120.1517938940677506No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG52890.1511366539390687No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52780.15082232170361215No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52300.14945069013071077No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA51460.14705033487813338No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA51330.1466788513271393No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC50330.14382128555026144No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG49960.14276398621281663No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.14153523292875916No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG48670.13907772636064425No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA47770.1365059171614542No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46430.1326767790204379No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT45840.13099081521207995No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA44290.12656158825791933No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT43010.12290390406351569No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA42650.12187518038383965No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA42490.12141796985953922No Hit
GAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.1141597527862695No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA38920.11121646003608533No Hit
GAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA37980.10853034820582017No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA37410.1069015357129998No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA37230.10638717387316181No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC36600.10458690743372877No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT36550.10444402914488488No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA35470.1013578581058568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTTC1700.030.4705893
CGTTATA1300.027.0384622
ATAAGTT2050.025.2682912
CCGAGTT451.3239677E-424.66666613
GTTATAC1950.023.7179493
AAGTTCC2350.023.617024
CGGTAAG400.001931972523.12528
TCGGTAA400.001931972523.12527
AAAAGTA21550.022.8352682
AAAAAGT22150.022.0496621
GCGGAAT1059.840733E-1021.14285919
CACGCAG4200.020.7023835
CGAGTTC450.003827348420.55555514
TACGGTG450.003827348420.55555528
CGTAGCG1101.7589628E-920.18181815
TCGTAGC1101.7589628E-920.18181814
GATACAT5300.019.8962251
TCGCCGG759.274658E-619.73333217
GAGGACT19450.019.4035991
GATAATG3950.018.7341771
Produced by FastQC (version 0.11.5)