FastQCFastQC Report
Thu 28 Apr 2016
ERR1041725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682885
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72651.0638687333884915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.8824326204265727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52030.7619145244074771No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA40950.5996617292809185No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32430.4748969445807127No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28030.41046442666041866No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT22990.3366599061335364No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.31557290026871293No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.30283283422538204No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG18360.2688593247764997No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC16190.2370823784385365No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15620.22873543861704387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14350.21013787094459535No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC12790.18729361459103655No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.16444935823747778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10800.15815254398617629No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10710.15683460611962483No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT10590.15507735563088954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.15302723006069835No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG9710.14219085204683074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9100.1332581620624263No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGT8670.12696134781112486No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT8110.11876084553036016No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.11846797044890428No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG7950.11641784487871311No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC7910.11583209471580135No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7690.11261046881978665No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6990.10235984096883077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA200.001841412537.013
AATAGTG809.767973E-1025.4375025
TAGACTA451.3224439E-424.6666665
CTAGACT400.001930388323.1250024
ACAGCGT400.001930388323.1250028
TCTAGAC753.7361679E-722.23
ATTAGAT502.7005465E-422.1999993
TCCGACC1059.804353E-1021.1428578
CCGACCA1059.804353E-1021.1428579
CCATAGG450.003824241520.5555552
GTGTATA1351.8189894E-1220.5555539
TAGACAG555.140155E-420.1818185
TTCTCGA1202.382876E-1020.04166823
AAGACGG3600.018.55
CGACCAT1103.844798E-818.510
GTCAGAT1409.276846E-1118.52
GTATAAG1700.018.511
TTACACC801.6151043E-518.54
TGGACTA500.007032030718.4999985
ACCCGTG500.007032030718.49999822
Produced by FastQC (version 0.11.5)