FastQCFastQC Report
Thu 28 Apr 2016
ERR1041723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7557719
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT397820.5263757490851406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT351000.464425840653774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172820.22866687687118295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158520.20974582410380696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115650.025.1305681
GTATCAA155650.018.6842272
AGGTCGC14950.014.84949835
CGAACTA13600.014.82720624
GGGTAAG11150.014.7668171
GTCGCCC15150.014.04290537
ACGAACG2900.014.03448329
TATACTG15350.013.8599355
GGTCGCC15850.013.65615136
CTACACT15450.013.6504864
ATCAACG220300.013.4026334
TCAACGC223000.013.2237665
TACACTG23650.013.2198735
TCACGTA11900.013.05882325
AACGCAG225900.013.0376277
CAACGCA227200.012.9874556
TGCTCGG20400.012.87745110
TAAGACT25400.012.8188974
GTTATAC8500.012.6235293
TATCAAC234200.012.6150723
Produced by FastQC (version 0.11.5)