FastQCFastQC Report
Thu 28 Apr 2016
ERR1041722.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041722.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916277
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230170.5877265576464585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT203160.5187579938804124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133410.34065516816098557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91580.23384454163993No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT78080.19937302698455703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77750.021.6289391
TTAACGG7150.018.6293735
TAACGGC7250.018.1172436
TACACAT22150.017.1218955
ATCTCCG20450.016.46454810
CCGTCTT7200.016.187537
TATACAC22250.016.0471923
GCCGTCT7500.016.03333536
AACGGCC8650.015.61271637
ACACATC23250.015.3569896
GTATCAA110250.015.3369612
ACATCTC25750.015.2310698
CTAGTAC2800.015.1964293
TTTAACG9000.014.834
ACGAGAC22700.014.58810522
AGGTCGC9400.014.56382835
TATGCCG8900.014.55056333
AATACTG14300.014.489515
GTATTAG16200.014.3888891
ATACACA28700.014.3745644
Produced by FastQC (version 0.11.5)