FastQCFastQC Report
Thu 28 Apr 2016
ERR1041721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455979
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152371.0465123466753297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141840.9741898749913288No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT91060.6254211084088438No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT56590.38867318828087494RNA PCR Primer, Index 26 (100% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.37919502959864115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42280.2903888036846685No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC39450.2709517101551602No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC36230.2488360065632815No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG32290.22177517670241123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19880.13654043087159912No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG16940.11634783193988375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32550.027.8494631
CCGTCTT8950.026.8715137
GTCGTAC358.870536E-426.4285721
GCCGTCT9200.026.34239236
TATACAC15950.024.4733523
TTATACA17150.022.4373172
TCACGTA1250.022.19999925
GTATCAA41700.021.649882
GTACACT1650.021.3030324
GTAGACT2350.020.4680864
TACACAT19800.019.9015165
ATATCGC851.2457767E-619.5882346
ATACACA20700.019.1256054
TGCCGTC13750.018.70181835
TGGACCG801.6171534E-518.55
ATAGACG500.00703576218.53
TACGCTG902.1530195E-618.55
CTTATAC20500.018.4097581
GTATACT2250.018.0888884
CGTATAC1054.79944E-717.6190473
Produced by FastQC (version 0.11.5)