FastQCFastQC Report
Thu 28 Apr 2016
ERR1041720.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041720.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5946415
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT359050.6038091858708146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT334550.5626078906366272No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT224490.37752158233153926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT215520.36243686321926744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137720.2316017297817256No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT70470.11850837857768085RNA PCR Primer, Index 17 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106200.022.6633721
TACACAT50350.021.3108255
CCGTCTT13850.020.70397237
ACACATC53050.019.3195116
GCCGTCT14750.019.31525636
ATCTCCG52000.019.1759610
ACATCTC57050.018.5486418
ATACACA63900.017.1971844
CACATCT61100.016.5621937
ATCTCGT57000.016.55263137
TATACAC63050.016.4607453
AATCTCG58000.016.3629336
CCCACGA60250.016.27385919
CGAGACA60400.016.23344223
ACGAGAC60400.016.23344222
TCTCCGA61400.016.1498411
TATGCCG19100.016.07853333
GCCCACG62750.015.71394418
CGAGCCC63050.015.63917515
GTATCAA155200.015.5438152
Produced by FastQC (version 0.11.5)