FastQCFastQC Report
Thu 28 Apr 2016
ERR1041719.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041719.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118113
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT16921.4325264788803942No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT4900.41485695901382574TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG4020.34035203576236317No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC3460.2929398118750688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2940.24891417540829544No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC2860.24214100056725338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.20234859837613134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1280.10837079745667284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC200.001835257537.04
TTATACA2200.031.1136362
CTTATAC2250.030.4222221
CACACTA250.005476643329.637
CAGTAGG250.005476643329.622
TATACAC2250.029.63
CTAGGAC250.005476643329.63
GGTATCA1600.027.7500021
ATACACA2700.025.3518524
AATCTCG551.8839874E-523.5454546
TACACAT2950.023.203395
TACTATC502.682262E-422.27
AAATCTC603.689977E-521.5833345
ACACATC3200.020.81256
GACACAT450.0038058220.55555726
TAAACCT450.0038058220.5555574
GTGACAC450.0038058220.55555724
GAGGCAC450.0038058220.5555577
GTATTAG555.105637E-420.1818181
ATTAGAG555.105637E-420.1818183
Produced by FastQC (version 0.11.5)