FastQCFastQC Report
Thu 28 Apr 2016
ERR1041718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10365149
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT601520.5803293324582213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT586560.5658963513211436No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT376980.36369954739676197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT366160.3532607201305066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286470.27637808197450897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111270.10735012106434746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166350.026.7796821
TACACAT100500.023.451745
ACACATC106750.021.541456
TATACAC109700.021.164543
ATACACA119000.020.365554
ATCTCCG110250.020.35419510
ACATCTC114350.020.3200728
GCGAATC105900.019.65297533
ATCTCGT85050.019.40270437
AATCTCG96650.019.2560836
GGCGAAT110150.019.213832
CACATCT118550.019.2100377
TCTCCGA117500.019.12978711
ACGAGAC116950.019.09320322
GACTAAG117400.018.97274426
CCCACGA118850.018.75683419
CGAATCT110600.018.73417734
AGACTAA119950.018.6002525
CGAGACT121450.018.5990123
CGAGCCC120850.018.56888615
Produced by FastQC (version 0.11.5)