FastQCFastQC Report
Thu 28 Apr 2016
ERR1041717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7953066
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC557240.7006606005784436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT547440.688338308772994No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG449060.5646375875668579No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406560.5111990771860814No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA395910.4978080151730163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308330.3876869624871716No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC260600.3276723718877726No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG240400.30227336224796825No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG232030.29174911914474244No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG227200.28567598961205654No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT224990.2828971870722562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217630.2736428944510205No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA188850.2374555925978736No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA163840.2060086009596802No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT134110.16862679122743354No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124640.15671943373788172No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT120730.1518030907828503No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC118090.14848361625566794No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC117910.14825728844699643No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC109430.13759473390513796No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC106790.13427525937795562No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG105690.13289214499162963No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA99410.12499581922242316No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93440.11748928023481763No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG90110.11330221577439442No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC84710.10651238151424872No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT84550.10631120123987405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT82210.10336893972714423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC81790.10284084150691067No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80630.10138228451769418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5600.017.83928535
TAACGGC5950.016.78991736
TATAACG13650.015.3150192
TATACTG9750.014.4205125
TTAATAC8400.014.3154773
TAATACT15250.014.31475454
GTATAAC16550.014.3081561
TCGTTTA10500.014.09523830
ATAACGC27000.013.909263
TCGAACG5350.013.8317763
ATTAGAG14450.013.8269893
CGAACGA5600.013.54464216
CGAACGT5600.013.2142854
TAACGCA17550.013.0712254
AAGTACT45350.012.9316434
GTATTAG25450.012.7210221
TAATACC8450.012.6982244
AAAGTAC39100.012.6803073
TAGACTA5600.012.5535715
TATACCG3850.012.4935065
Produced by FastQC (version 0.11.5)