FastQCFastQC Report
Thu 28 Apr 2016
ERR1041716.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041716.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245161
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA46881.9122127907783049No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41451.6907256863856812No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28441.1600540053271116No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG18520.7554219472102007No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.6110270393741256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC14820.6045007158561109No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13750.5608559273293876No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13450.5486190707331101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.5453559089741027No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13240.5400532711157158No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT12740.5196585101219199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12470.5086453391852701No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11730.47846109291445216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10840.44215841834549546No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT10740.4380794661467362No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC9900.40381626767715906No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9060.36955306920758196No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9040.3687372787678301No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8500.34671093689453053No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7110.2900135013317779No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.25860556940133217No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC5760.23494764664852893No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5740.2341318562087771No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5560.22678974225101056No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5470.2231186852721273No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA5320.21700025697398853No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.2141449904348571No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG4810.1961976007603167No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4330.1766186302062726No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCT4120.16805283058887832No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4000.16315808795036732No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT3990.1627501927304914No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.15989492619135998No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3820.15581597399260078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3820.15581597399260078No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3720.15173702179384158No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3490.1423554317366955No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.14153964129694363No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.13134226080004568No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC3030.12359225162240324No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGT2880.11747382332426447No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2860.11665803288451262No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2810.11461855678513302No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC2740.11176329024600161No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2640.10768433804724242No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.10197380496897958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA2470.10075011930935182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT200.001839113437.06
TATCAGT250.00548801629.64
AAACTCA250.00548801629.618
TTAAGAC250.00548801629.63
GATTATA250.00548801629.62
GTCTCGT453.9913684E-628.77777934
GACGGAC950.027.2631597
CAGAGCG950.027.26315914
TCGTTCG358.849423E-426.4285737
TATTCTG451.3190678E-424.6666685
GCGAAAG1051.8189894E-1224.66666618
GAGCGAA1051.8189894E-1224.66666616
ACGGACC1051.8189894E-1224.6666668
AGCGAAA1051.8189894E-1224.66666617
CGAAAGC1150.024.13043219
TCTCGTT551.8947543E-523.54545435
CGGACCA1103.6379788E-1223.5454549
CTCGTTC400.0019268823.12536
CGCGCAA400.0019268823.12521
GCGCAAA400.0019268823.12522
Produced by FastQC (version 0.11.5)