FastQCFastQC Report
Thu 28 Apr 2016
ERR1041715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052433
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC392900.7776451464076812No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA310820.6151887615333048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304560.6027986912443964No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG301830.5973953538819812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230110.45544394156241164No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC208890.41344437422524954No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG186470.36906971354197077No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG181300.35883701970911835No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT177600.35151381522525876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168310.333126634237406No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG162680.3219834879552089No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA118300.23414461903799613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107820.21340213714857773No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA102180.20223919842182964No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94710.18745424234225372No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC91770.18163526364426802No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC86840.17187758848063892No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT82250.16279285643174288No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC82190.16267410176443706No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG80910.16014066886191267No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC80410.15915104663436408No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA71560.14163473320675404No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68620.13581575450876834No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG68580.13573658473056446No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT68090.13476675494756685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC65030.12871026691496948No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC63980.12663206023711746No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG63820.12631538112430188No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC56060.11095644415274779No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.1104022557053206No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT54940.10873969036303895No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT52280.10347490011248046No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG51030.10100084454360898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1451.5643309E-1017.8620682
TACGTAG1851.8189894E-1116.014
TATAACG8000.015.9562512
ATTAGAC2500.015.543
TTAGACT3700.015.54
GTACGTA2055.456968E-1215.34146313
TAATACT10700.015.2149524
TATACTG6450.015.201555
GTATAAC9700.015.067011
AAAAGTA29900.014.7257522
AGTACTC29550.014.587145
AAAGTAC30250.014.4330593
CGATAGT1304.4508706E-614.23076928
AAGTACT33850.014.1004444
AAGACGG11700.013.9145295
TATACCG2151.9826984E-1013.7674425
AAAAAGT30950.013.568661
ACGGACC11250.013.3200018
GACGGAC10750.013.2511637
TATCACG6400.013.00781252
Produced by FastQC (version 0.11.5)