FastQCFastQC Report
Thu 28 Apr 2016
ERR1041714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705264
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA99291.407841602577191No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG85011.2053642324009164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81831.1602747340003177No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT81591.1568717529889516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80981.1482225095850631No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG67170.952409310556047No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG63290.8973944508722974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50900.72171555616053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC49390.7003051339640192No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC44920.6369246126273282No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT35010.4964098550330089No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC33760.47868599559881125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG33020.46819347081376617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC31580.44777558474557044No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28850.40906667574128264No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG27190.38552939041266815No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26940.3819846185258286No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG25040.3550443521858481No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT23530.33363392998933733No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA23260.3298055763515506No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22710.32200707820050367No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG19900.28216384219242724No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC19340.27422355316590663No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.26897729077338417No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG18810.26670863676580686No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC18590.26358923750538804No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA18250.2587683477392863No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.25054447696181853No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC15710.22275346536899657No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC15650.22190272011615506No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC15550.2204848113614193No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15460.21920869348215702No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT15350.21764899385194764No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA15180.21523854896889674No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT15180.21523854896889674No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.20701467819142905No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.2034699063045895No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG13390.18985798225912567No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12840.18205948410807868No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA12680.17979083010050137No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT12620.17894008484765989No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12160.17241770457587513No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.1556863812699925No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10970.15554459039451893No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10900.15455205426620386No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10830.1535595181378888No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10820.1534177272624152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10660.1511490732548379No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10450.1481714648698927No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10420.14774609224347193No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT10160.14405952948115883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10150.14391773860568524No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9680.1372535674584269No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9670.13711177658295332No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT9650.13682819483200617No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9610.13626103133011186No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT9350.13257446856779873No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8900.12619387917148756No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT8470.12009687152612356No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.1198132897751764No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8130.11527598176002177No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7780.11031330111844642No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG7650.10847001973728987No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.10747748360897479No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG7570.10733569273350121No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA7510.10648494748065973No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT7380.10464166609950316No Hit
GAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7370.10449987522402958No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.10393271172213527No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.10138047596361079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT250.005494920529.64
TTCGTCT250.005494920529.622
CGTCCTA250.005494920529.633
TACACTC250.005494920529.65
GGGTATT358.8659837E-426.428571
CGTTATT358.8659837E-426.4285733
GTTATAC451.3225038E-424.6666683
AAAAAGT6300.024.0793671
CTCACCT400.001930451323.1259
CGACCGG400.001930451323.12515
AGTATCC400.001930451323.1253
GTATACT400.001930451323.1254
TCTCGAT502.7006672E-422.224
TATAGTG502.7006672E-422.25
AAAAGTA6950.021.827342
GCTAATA855.1735697E-821.7647062
CTTACAC603.7236598E-521.5833323
GACGGAC806.9522684E-720.81257
CGGACCA806.9522684E-720.81259
ATCCTAA450.003824363720.5555571
Produced by FastQC (version 0.11.5)