FastQCFastQC Report
Thu 28 Apr 2016
ERR1041713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822819
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC731741.072489245281166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA544890.7986288365556817No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG501080.734417841071264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368570.5402019311958884No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC351030.5144940822847565No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG307270.4503563702921036No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT303360.4446256012360873No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG299400.4388215486883061No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276940.4059026041875066No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG263870.3867462994401581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205380.3010192707735615No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA200850.2943797864196602No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT162450.23809806474420617No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC159910.23437526336254852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT147830.21666997175214525No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC147290.2158785100410842No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC142590.20898986181518225No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT141390.20723105801282432No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA133150.19515393856996646No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG132430.19409865628855172No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA131570.19283818023019517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC119640.17535273909508664No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT119240.17476647116096733No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG115870.16982716381601212No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC111980.1641257081567018No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG110170.1614728457548119No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT103730.15203393201549095No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT100350.14707996797218276No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC95050.13931191784510186No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG93490.13702547290203654No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86450.1267071572615366No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT84110.12327748984693865No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA84070.1232188630535267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82520.12094707480881435No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG80540.11804504853492376No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC80160.11748809399751041No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT78450.11498179857915036No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT74120.10863544819230879No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG73090.10712580826195155No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG68700.1006915176849921No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68410.10026647343275559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG10850.016.5391712
GTATAAC13200.016.2575761
AAAAGTA45750.016.1748622
AAAAAGT47150.015.3806991
AAGACGG14500.015.3103445
GATACAT9400.014.9574461
TTAACGG3600.014.90277835
TCGAACG4350.014.88505653
CGTTATA2750.014.82
CGAACGT4400.014.7159094
AAGTACT55850.014.5085054
AAAGTAC52550.014.3986683
AGTACTC52700.014.2874775
CGCAAGA15450.014.1294492
ATAACGC21700.014.066823
CGTTAAT2551.8189894E-1213.7843131
CACGCAG9500.013.6315785
ATTAGAG11550.013.6147183
GACGGAC15500.013.3677427
GTACTCC29100.013.35051536
Produced by FastQC (version 0.11.5)