FastQCFastQC Report
Thu 28 Apr 2016
ERR1041711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6255046
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1425302.278640316953704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1046531.673097208237957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT832781.3313731026118754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT603340.9645652486008895No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC545190.8716003047779345No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA392100.6268539032326861No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA388620.6212903949867036No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG387580.6196277373499731No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316560.5060874052724792No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG265390.42428145212681084No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG244610.39106027357752443No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC243990.3900690738325505No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT237430.37958154104702024No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204920.3276075028065341No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202080.32306716849084727No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT190310.30425036042900405No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG189470.302907444645491No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA175570.2806853858468827No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC123350.19720078797182305No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121920.19491463372131876No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA118210.18898342234413623No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA118030.18869565467624058No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT116750.1866493068156493No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113560.181549424256832No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA112680.1801425601026755No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC109440.17496274208055385No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC99210.1586079462884845No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG96460.15421149580674545No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA94470.15103006436723246No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT94200.150598412865389No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA93040.14874391011672816No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89940.14378791139185867No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88650.14172557643860653No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA86230.13785670001467615No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA84520.1351229071696675No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84220.1346432943898414No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT83400.13333235279165012No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA79820.12760897361905893No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC79810.12759298652639806No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG79200.12661777387408502No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA79190.12660178678142414No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC77520.12393194230705896No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT77510.1239159552143981No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA76470.1222532975776677No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG74710.11943956926935469No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC73390.11732927303811994No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA72450.11582648632799822No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA69600.11127016491965047No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69510.11112628108570265No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA69170.11058271993523308No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65330.10444367635345927No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA64170.10258917360479843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA2650.019.547172
TGCGCGA1002.880006E-718.512
CGCGATA902.1553296E-618.49999814
CACGCAG8450.018.3905335
ATAGTCG1054.805697E-717.61904730
GTCGGGA2850.017.5263162
GTACTAG2150.017.2093031
TTAACGG2300.016.89130435
AAAAGTA31400.016.4968152
TGCGACG1352.224624E-816.44444522
CGGTCCA4800.016.187510
CAATGCG1504.6820787E-916.03333319
TAACGGC2450.015.85714336
CGTAACG700.002594220715.85714223
GATACAT7250.015.3103451
GTCTTAC2300.015.2826071
CTGTGCG1954.1836756E-1115.1794879
TAAGTTC3450.015.0144933
AAAAAGT34250.014.908031
GATACAA9100.014.8406591
Produced by FastQC (version 0.11.5)