FastQCFastQC Report
Thu 28 Apr 2016
ERR1041710.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041710.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6413451
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1769682.75932567349466No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1250471.9497615246456237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1107561.7269329725915112No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC811531.265356202144524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT805201.2554863208590819No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG701251.093405095010471No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG674521.0517270655065425No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT668641.0425588345494492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT642291.0014733097672377No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG571290.8907684801832898No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA484670.7557085880908734No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG459700.7167747909822653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT440240.6864323123385522No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC364800.5688045328482279No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT363460.5667151740926998No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC351760.5484722655556268No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC338270.5274383479346767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT336280.5243354942604224No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC334220.521123494979536No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT322730.5032080232623591No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA318600.4967684324710674No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC293940.4583179944775442No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA293060.456945878279884No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG275910.4302052046550289No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT268700.41896320717192664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC266400.4153769943825875No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG250530.39063212613614734No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC243740.38004500229283733No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT236580.36888096595732933No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT219810.34273279705419124No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219690.3425456902999649No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217680.33941165216667285No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT211400.32961973202882505No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG208540.3251603543864294No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA195750.30521789283179995No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT181960.2837162083252838No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG176870.2757797635001811No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT173800.2709929490378893No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG173340.2702757064800214No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG164420.2563674377491931No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG158210.24668466321797733No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG157630.2457803139058831No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151450.236144316063224No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151010.23545825796439387No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT150350.23442917081614875No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT143910.2243877750059991No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG139940.21819765988700937No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT139590.21765193185384904No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC131430.2049286725664545No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129040.20120212971144552No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT124810.19460661662496526No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT123940.1932500926568239No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT123330.19229896665617308No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA122610.19117632613081476No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA121060.18875953055539055No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120130.187309453210136No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT119270.18596852147151355No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC116610.1818209884194952No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT116240.1812440759272972No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA115310.17979399858204265No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA113290.17664436821923174No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT103670.16164464342208273No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102790.16027252722442253No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT102470.15977357587981883No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT100450.15662394551700792No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA97300.15171239321856517No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT96360.15024672364379177No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC89830.14006499776797235No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG87840.13696214409371804No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA87170.13591746471595403No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA86160.13434264953454855No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA82860.12919721379332283No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82510.1286514857601625No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA82390.12846437900593613No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA81190.1265933114636722No Hit
CTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA80510.12553303985638933No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACA79620.12414533142921025No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC79610.1241297391996914No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC79180.12345927333038016No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77970.1215726135585974No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG77650.12107366221399368No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77630.12104247775495595No Hit
CCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA77340.12059030309890884No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA76420.11915581798317319No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT75910.11836061427771102No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA75630.11792403185118278No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA75390.11754981834272998No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA74750.11655191565352258No Hit
CCACTCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT74070.1154916440462397No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT72240.11263826604428723No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA71350.11125055761710817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA71180.11098548971528745No Hit
ATACAACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC70470.10987844141944797No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG69870.10894290764831602No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT68010.10604275295780696No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG67800.10571531613791077No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA67690.10554380161320324No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT66040.10297108374259038No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC64480.1005386959376473No Hit
TACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC64320.10028922026534544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAGT5500.031.6181831
ATAAGTT5350.031.1214942
TAAGTTC5450.030.2110083
GATAATG6250.029.8961
GATACAT12150.029.8436221
AAGTTCC6550.025.1374054
GATAATC4000.024.9749981
CACGCAG10050.023.930355
GATAAGC3300.023.5454541
AAAAAGT74600.023.5341821
AAAAGTA74900.023.5140172
TACATTC9650.023.0051823
CGTTATA3400.022.8529422
GATACAA12900.022.5155051
ATACATT12050.022.414942
TAAGCTT3500.021.6714293
GATACAG5000.021.461
CGCAAGT3800.021.4210531
ATAATGC3200.021.3906272
ATAATCT4850.020.5979392
Produced by FastQC (version 0.11.5)