FastQCFastQC Report
Thu 28 Apr 2016
ERR1041709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8121531
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC759060.9346267347868278No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG539200.6639142299647689No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA530460.6531527122164528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT493060.6071022815772051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394820.48613986697828276No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC329280.4054407968152803No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG315430.3883873619395161No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG294920.3631335027841426No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT291230.35859002446706173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286590.35287681595994647No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG269440.33176010779248394No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA212760.26197031077022304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT212570.2617363647322161No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA181420.22338152744845766No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT162320.1998637941540825No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC152840.1881911181524764No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC152060.18723070810171139No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC146670.18059402839193744No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG146020.17979368668296655No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT142620.1756072838975804No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC130760.161004125946204No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128630.15838146773065326No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA124620.15344397503377136No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG114470.1409463314244568No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110360.13588570923388707No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT110050.13550400780345478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC108930.13412495747415112No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG107010.1317608711953448No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT104030.12809161228344754No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC102930.12673718785288143No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC91990.11326682124343304No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90520.11145681768622198No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT83980.10340414879903802No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT83600.10293625672302426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG15700.015.7898092
ATAACGC25550.015.7847373
AAAAGTA41900.015.1885432
GTATAAC19050.014.8582681
TTAACGG4000.014.33750135
AGTACTC48100.014.0769235
GATACAT10900.013.9174311
AAAAAGT44600.013.89574051
TCGTTTA9450.013.70370430
AAAGTAC48250.013.6880833
TATACTG10150.013.4876835
AAGTACT55250.013.4606334
TAACGCA19600.013.4030614
TAACGCC10450.013.1004794
TAACGGC4550.013.01098836
GACGGAC13250.012.9849057
AATAACG10850.012.9585262
CGTTATA3150.012.9206352
ACGGACC13750.012.7818188
CACGCAG14150.012.5512365
Produced by FastQC (version 0.11.5)