FastQCFastQC Report
Thu 28 Apr 2016
ERR1041708.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041708.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15742071
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC561910.3569479517656857No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA383240.24344954358292503No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG368190.23388917506470397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239190.15194315919423818No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC215800.13708488546392655No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG201170.1277913179276094No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT201120.12775955590595417No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG195770.12436101958884571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191980.1219534583473801No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG181500.11529613860844612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173030.10991565214005197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG10800.016.6157422
TTAACGG10100.016.30198135
TAACGCA13850.015.7617334
TATACTG23400.014.8632475
CGAACGA16600.014.2650616
TAACGGC12050.013.81742836
ATAACGC38700.013.7196393
GTATAAC19400.013.1597941
TAGACCG2152.910383E-912.9069775
TTAGACT14500.012.7586214
TACCGTC27500.012.7145467
TAACGAA18750.012.629333513
GTATTAG55750.012.5766811
TAATACT40700.012.5454554
ACGAACG18750.012.53066715
AAGACGG40650.012.4698655
ATAACGA19400.012.39690712
CCGATAA19150.012.26892959
ATTAGAG37850.012.2681633
TAGAGTG26250.012.26285655
Produced by FastQC (version 0.11.5)