FastQCFastQC Report
Thu 28 Apr 2016
ERR1041707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9424390
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1486271.577046365865589No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1050681.1148519957259833No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1018631.0808444896698883No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC656650.6967559704129391No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG614460.6519891473082078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT579340.6147241359918254No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT578790.614140543844217No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG577760.6130476349132411No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG512760.5440776538322374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451950.4795535838393785No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA416970.4424371232514783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT330480.3506646053484629No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG325050.344902959236619No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT322190.3418682800690549No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC307990.3268009918944356No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC305460.32411646801543653No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC291370.30916589827033897No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT274360.29111698475975634No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC273310.29000285429614014No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG256380.2720388269161187No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA240230.2549024393090693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238820.25340632125792756No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA222450.2360364967918348No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT221930.23548473694318678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC219890.2333201406138753No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG217170.23043401217479326No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC203770.21621558530578638No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC203000.21539855629913446No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT195300.20722826623261556No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT191290.20297334893823368No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG180130.19113173372494136No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT164300.1743348906401369No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA163970.17398473535157183No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG160520.17032402097111854No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG153750.163140532172374No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT152180.1614746418601098No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144810.15365450708215597No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144670.1535059563536738No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG139540.1480626332314346No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT136760.14511284019443169No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG131890.13994539699651648No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT131490.1395209663437103No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126760.1345020738742773No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT122690.13018349198197443No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT115590.1226498478946648No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG115540.12259679406306404No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC115410.12245885410090201No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113130.12003959937990681No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG107460.11402329487637927No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT101450.10764622431796646No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA100710.10686102761027505No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT98240.10424016832919691No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA94940.10073861544354595No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT94330.10009135869801652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA76800.019.2467442
AAAAAGT78100.018.8553141
CACGCAG12900.017.2093035
CGTTATA3450.017.159422
TATAACG18100.017.0690612
GTATAAC20150.016.342431
GATACAT13950.015.9139791
AAAGTAC98850.015.0844713
AAGTACT106000.014.9396224
AGTACTC102900.014.8323625
ATAACGC30650.014.7879283
TATACTG8750.014.3771435
CTAGTAC4050.014.1604943
TAACGCA23400.014.0726494
TACGGGT2500.014.0599994
CGAACGT5150.014.0097084
TAATACT13700.013.9087594
GATACAA16200.013.7037041
TATCACG15300.013.6633992
TCGAACG5300.013.6132083
Produced by FastQC (version 0.11.5)