FastQCFastQC Report
Thu 28 Apr 2016
ERR1041706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9049797
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1296801.4329603194414195No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG908781.0041993207140447No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA898510.9928509998622069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT589180.6510422277980379No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC582170.6432961976937163No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG537890.5943669233685573No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT507200.5604545604724614No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG503470.5563329210588923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492750.5444873514842378No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG462710.5112932367433215No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA357580.3951248851217326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT342160.3780858288865485No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT286410.31648223711537393No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG272400.30100122687834874No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC269940.29828293386028437No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC266660.2946585431695319No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC253970.28063612918610215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT244780.2704812052690243No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT244400.27006130634753467No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA236680.26153072825832446No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC230560.2547681456280179No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG215220.23781748916577908No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA213540.23596109393393022No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT193950.21431419953397848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC190890.21093290821882524No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG187330.2069991183227646No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC176120.1946121001388208No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT175360.19377230229584155No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC166310.1837720779814177No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159790.17656749648638528No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT157790.1743575021627557No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG157710.1742691023898105No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146490.16187103423424856No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT142310.15725214609786275No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA140180.15489850214319725No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG139750.15442335336361687No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG133030.14699777243622147No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT129380.1429645327955975No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG125580.1387655435807013No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124590.13767159639050466No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT119270.13179301148964997No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116970.12925151801747597No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT115450.12757192233151748No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG111530.12324033345720352No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT105890.11700814946456811No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC103420.11427880647488556No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT99410.10984776785600825No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG97340.10756042373105165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA66400.018.722892
TATAACG18550.018.6495952
AAAAAGT69050.017.6560461
GTATAAC21550.017.1693741
GATACAT12750.016.3960781
ATAACGC29350.015.6320283
CACGCAG11700.015.3376075
GTACTAG3500.015.3285721
AAAGTAC82550.015.1496063
TAACGCA24100.015.0456434
AAGTACT90100.014.8041074
AGTACTC86250.014.6069565
GATACAA15400.014.0551941
TATACTG9900.014.0151525
TATCTAG10350.013.584541
CTAGTAC4000.012.9500013
GTACTCC43400.012.91589836
GTATTAG17300.012.832371
CGTTAAT3500.012.6857151
GTACGTA2204.096364E-912.61363613
Produced by FastQC (version 0.11.5)