FastQCFastQC Report
Thu 28 Apr 2016
ERR1041705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7970776
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1187591.4899302150756715No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA878991.102765903846752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG837451.05065052637284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT537820.6747398245791878No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG525280.6590073538636639No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC523130.6563100004315765No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG503830.6320965486923733No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT496490.6228879095335259No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG455880.5719392942418655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420260.5272510480786312No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA344080.43167691577331996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT299800.37612398090223587No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT278540.34945154649936216No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC261900.32857528551799725No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG255820.32094742092865236No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC248350.3115756859808882No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC245300.30774920785629906No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT238320.29899221857445246No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC236500.29670887752961567No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG214270.2688194976248235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208010.2609658080969782No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA202080.25352613095638366No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT190190.2386091391854444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC189330.23753019781260948No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA186180.2335782613888535No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG183340.23001524569251477No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC178030.22335341000675468No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC166040.20831095993664858No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT155080.1945607303479611No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT154770.19417180962054384No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG150370.18865164445720214No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA138240.17343355276826247No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG136200.1708742034652586No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT129340.16226776414241223No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128550.16127664357899407No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT121820.15283330004506462No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT119820.15032413406172748No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG118800.14904445941022554No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117640.14758914313989002No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG116450.14609618937980443No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG113840.14282172777154947No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT105920.13288543047753443No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC102100.12809292344936052No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT100710.12634905309094122No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG100130.12562139495577346No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99000.12420371617518795No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98100.12307459148268626No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT93870.11776770542792822No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA89970.1128748317604208No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG89310.11204680698591955No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT85580.10736721242699582No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT84070.10547279210957629No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81630.10241160960990497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT65600.018.6692071
AAAAGTA67350.018.4038622
TATAACG16000.018.1531262
TAAGTTC7000.017.7071443
GTATAAC19150.016.7127931
GATACAA14400.015.6736111
ATAACGC24100.015.6597513
GATACAT11150.015.5964131
AAAGTAC82500.015.2260613
CGTTATA2900.014.6724142
AAGTACT91250.014.6175354
TAACGCA20250.014.6172844
AGTACTC87850.014.5725685
GTATTAG17300.014.329481
CACGCAG11750.014.327665
ATTAGAG10350.014.1207733
TAATACT17150.014.0233234
GTACTCC39600.013.73484936
TAGACTA7200.013.6180555
GATAAGT9850.013.5228431
Produced by FastQC (version 0.11.5)