FastQCFastQC Report
Thu 28 Apr 2016
ERR1041704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4955879
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1239882.501836707474093No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA895811.807570362391818No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG822841.660331093636467No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC578231.166755685520167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT543511.096697477884347No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG521961.0532137689398793No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT489500.9877158017780499No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG487060.9827923563105556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473880.9561976795640087No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG412480.8323044206688662No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA349760.70574765848803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300460.6062698463784124No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG274850.5545938470249172No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT265360.5354448726451957No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC256630.5178294304602674No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC249940.5043303115350476No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC246600.49759084110003493No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT226800.45763829181463067No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA220570.4450673634283646No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC218370.44062819128554187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214290.4323955447661253No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA213770.4313462858960035No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG195950.39538899153913964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC188620.3805984770814622No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT188380.38011420375679067No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG181340.36590885289975805No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC174860.35283347313362573No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC172480.34803109599729937No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT159630.32210229507217586No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159080.32099250203647023No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT154460.3116702405365426No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152850.3084215736502041No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG152710.30813908087747904No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA138560.2795871327770513No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT130550.2634245105661377No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT126100.2544452760045191No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG124670.2515598141116843No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG124470.2511562530077914No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115430.232915291111829No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG113690.22940430950796012No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT112190.22637760122876283No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG111110.22419837126774078No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110140.22224109991385987No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT105450.21277759202756968No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT104490.21084049872888339No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG99300.20036808808286077No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC96270.19425413735888225No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT91770.18517401252129037No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG90480.1825710434011807No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT90180.18196570174534124No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.18188498952456264No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA87490.17653780489798077No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT86750.17504462881357674No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA86000.17353127467397814No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT81980.16541969648572938No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA80500.16243334431692139No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA78990.15938645798252943No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT78860.159124143264999No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA77180.15573422999229802No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT76880.15512888833645858No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT75460.15226360449881848No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC72790.14687606376184728No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71620.1445152313040734No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT68380.13797754142100724No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC67850.13690810449569088No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACA67760.13672650199893904No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT67570.1363431189502407No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA65130.13141967348274647No Hit
CTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA64270.12968436073600667No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA64080.12930097768730836No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA60710.12250097308671176No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT59110.11927248425556798No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC58490.11802144483349977No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA58240.11751699345363355No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA57360.11574132459650446No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA56970.11495438044391319No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55860.11271461631730717No Hit
CCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA55700.11239176743419281No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG55400.11178642577835335No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA54720.11041431802511724No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54480.10993004470044568No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT53370.10769028057383968No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53360.10767010251864503No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC52630.10619710448943567No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA52510.1059549678270999No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG52200.10532944811606579No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52050.10502677728814605No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA51370.10365466953490994No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG51250.10341253287257417No Hit
ATACAACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC50740.1023834520576471No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT49810.10050689292454477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAGT5250.026.076191
TAAGTTC5200.025.6153853
GATACAT7250.024.7517221
AAAAGTA49500.023.8444442
ATAAGTT5550.023.6666662
GATAATG5250.023.2571451
AAAAAGT51300.022.8635481
GATAAGC2200.021.8636361
CACGCAG8100.021.240745
TGCGACG1101.7589628E-920.18181822
GATACAA10400.020.1009621
AAGTTCC6900.019.5724644
GATACAC7550.019.3576151
TATAGAC1153.0577212E-919.3043483
CGTTATA2450.018.877552
TATACTG1501.2732926E-1118.4999985
GATAATC3050.018.1967221
AATGCGA1552.1827873E-1117.90322720
CGCAAGT3100.017.9032271
CCACTCT19300.017.7331621
Produced by FastQC (version 0.11.5)