FastQCFastQC Report
Thu 28 Apr 2016
ERR1041703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727393
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1095482.317302580936258No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA795231.6821745092908502No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG793801.679149586251873No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC452760.9577371714177348No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG451100.9542257222955655No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT444940.9411952845892018No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG426970.9031827901763191No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG424290.8975137036417323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT392290.8298231181541285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308400.652368017636782No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA301580.6379414616047365No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT261240.5526090172744259No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC237930.5033006564082995No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC237650.5027083637852829No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC223600.4729879660946319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221130.4677630990273075No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT216280.4575037446643425No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA195820.4142240765682058No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG182810.3867036229059018No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG174430.3689771508313356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC172510.36491571570207937No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT170770.3612350401161909No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG169350.3582312703851785No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC166430.35205450445943465No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC165280.34962187404347383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT149890.31706693308552936No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT138450.292867548773711No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG137160.290138772046242No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA125830.2661720741220372No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA125750.2660028476583182No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC121950.25796459063166527No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT121540.25709730500510536No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG116140.2456745187040722No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG115990.24535721908459907No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT115610.24455339338193377No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG112140.2372131955181217No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT100560.21271766489479507No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG100240.21204075903991904No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT94750.20042759296720203No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC94460.19981414703622058No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.198375722094609No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT91410.19336238810693335No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT89070.18841251404315235No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT81310.17199754706240838No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80360.16998798280574515No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA78740.16656114691543522No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT78020.16503810874196412No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT74400.15738061125867894No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG72370.15308648974180905No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC68620.1451539992549805No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67670.14314443499831725No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG66420.14050027150270772No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT64300.13601577021415395No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.13231394132030064No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60870.12876018558220143No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA59480.12581987577508366No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT58430.12359877843877165No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57040.12065846863165385No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA56980.1205315487838646No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT56590.11970656977323443No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT55990.11843737129534186No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC55870.11818353159976334No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC53950.11412209647050711No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACA51520.1089818426350422No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG50050.10587230636420537No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA48870.10337621602435No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.10214932416238717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT43000.021.6406971
AAAAGTA43450.021.5443022
GATACAT6500.019.9230791
TAAGTTC4300.018.9302333
CACGCAG6950.018.6330935
GATAAGT4800.018.1145841
TATAACG11150.016.7578472
GTATAAC11600.016.7456911
GATACAA9050.016.353591
TCGAATA803.3853523E-416.187513
GTCCTAA1951.8189894E-1216.1282061
AAAGTAC59000.016.022883
CGTTATA1751.3278623E-1015.8571432
CGAACTA700.00259409515.85714224
AGTACTC60800.015.8223695
CCACTCT14750.015.6779651
GATACAC7200.015.6736121
AAGTACT62550.015.6458844
CACTCTT15100.015.314572
ATAAGTT5000.015.172
Produced by FastQC (version 0.11.5)