FastQCFastQC Report
Thu 28 Apr 2016
ERR1041702.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041702.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15413824
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC644670.41824144352498127No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG597130.3873989997550251No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA428020.2776857968535258No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG312460.20271413505175612No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC250260.1623607483775603No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT208450.13523574682051642No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG193960.12583509452294253No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG181800.11794607230496469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG11600.016.10775835
TAACGGC12250.015.40408136
TATAACG6150.015.3414632
TCGTTTA29800.014.40268430
ATTAGAG34050.014.1806173
TATTAGA27850.014.1490132
TAATACT36950.013.9688774
ATAACGC40950.013.9597083
GTATTAG55850.013.8460171
AAGACGG47700.013.8459125
TATACTG21000.013.6547625
TAACGCC33100.013.6374634
CGAACGA22850.013.35886316
TTAGACT15200.013.2664484
GACGGAC45800.013.1681227
TACCGTC30500.013.101647
ACGGACC46600.013.0611588
ATTAGAC12450.012.9277113
TATACCG6550.012.7099235
CGACGGT35200.012.6661937
Produced by FastQC (version 0.11.5)