FastQCFastQC Report
Thu 28 Apr 2016
ERR1041701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424257
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA402380.6263448053214559No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG397870.6193245382306468No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC386400.6014703334564604No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG304060.47329986954133374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT279320.4347895795576049No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT256210.3988165479681153No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG247460.38519629585180043No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG240740.3747359422264707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204760.31872946552418435No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC179550.27948757342677916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160440.24974094280474768No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT146240.22763721937027115No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC124040.19308069400087824No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA124030.1930651279984596No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC120480.18753919713984046No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT116950.18204439828605862No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC109640.1706656505180288No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT96220.14977607527220657No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG95580.14877985111741326No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC95510.14867088910048273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC95180.14815721102066745No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC92220.14354967430474838No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG89000.13853742152594456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73830.11492379585685941No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA72850.11339832761983215No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG70740.11011390110949797No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA67890.105677590420184No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT65070.10128797773812598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG6200.015.8145152
CGAACTA2650.015.35849124
TACGACG11600.014.8318975
CGACGGT11550.014.735937
ACGACGG11850.014.5189876
AAGACGG15700.014.2579625
AAAAGTA37950.014.1857712
TAATACT11750.013.6978714
ATAACGC16500.013.6787883
AGTACTC41300.013.57263855
AAAGTAC40550.013.5499383
AAAAAGT39000.013.4717951
TACCGTC10950.013.3470337
GGTTATC8050.013.3291932
TAGACTA5000.013.325
GACGGTA13000.013.2346158
TAGACAG7850.013.1974535
TCTATAC7150.013.1958053
TATACTG8850.013.1694915
ACGGACC15400.013.0941558
Produced by FastQC (version 0.11.5)