FastQCFastQC Report
Thu 28 Apr 2016
ERR1041700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13417147
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1042610.7770728009464307No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG804590.5996729409016686No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA745770.555833516618697No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC457590.3410486595995408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT455030.3391406533743724No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG420600.3134794602757203No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG404440.3014351709793446No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT399900.2980514411893974No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG383480.285813370010778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357700.2665991510713865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT285340.21266816261310992No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA278600.20764473997340865No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT236320.17613282466086122No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC217540.16213581024341464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211400.15755957656273722No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC210420.15682916792966492No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT199570.14874250092065028No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC198570.14799718598894385No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG197760.14739348089426166No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC190120.1416992748160246No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA170880.12735941552999308No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA153870.11468160854166687No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG153200.11418224753742356No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT150500.11216989722181622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC148590.11074634570225697No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG147050.10959856070742909No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC144990.10806321194811386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG15200.014.4835512
TAATACT31000.014.0241954
GTATAAC19900.013.9447231
TCTATAC15750.013.50793653
TCGTTTA20000.013.50530
TACCGTC21700.013.3847927
GTATTAG40150.013.3163141
ATAACGC40750.013.3018413
TTAACGG7100.013.28873335
TATACTG17900.013.229055
ACCGTCG21100.013.1516588
AAGACGG31350.013.0414685
ATACCGT25300.012.8695656
ATTAGAG26900.012.8605953
TAACGCA18800.012.7925544
AAAGTAC63750.012.7686273
TAGAGTG20650.012.7215495
CGTCGTA20900.012.65789610
AGTACTC70000.012.6328575
CCGTCGT21750.012.50344859
Produced by FastQC (version 0.11.5)