FastQCFastQC Report
Thu 28 Apr 2016
ERR1041699.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041699.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574166
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC241800.9393333607855903No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA239660.9310199886099031No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG198140.7697250293881591No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG144530.5614634021271355No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG142240.5525673169484796No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT140870.5472452048546986No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG135540.5265394694825432No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC88010.34189714260851867No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75880.29477508443511413No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC72640.2821884835709896No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC71980.2796245463579272No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA71240.2767498288766148No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC65550.25464558229733436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61940.2406216226925536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61460.2387569410830537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC60290.23421177965989765No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT58920.2288896675661166No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG58790.22838464963021032No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC58240.22624803528599166No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG53280.2069796586544924No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT49440.19206220577849292No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC47070.18285534033158699No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG47060.18281649279805576No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC46520.18071872598736832No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG45150.1753966138935873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44700.1736484748846811No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT39110.15193270364071315No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA38910.15115575297008818No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG38310.14882490095821327No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG38310.14882490095821327No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT36970.14361933146502595No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT34640.13456785615224504No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA32800.1274199099824953No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG31470.12225318802283923No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30430.11821304453558938No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25990.10096473964771502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG500.007037194418.52
CTACCCG1409.458745E-1118.510
GTCGTAC852.7252392E-517.4117641
CGTTATA650.001580653417.0769232
CCGAGTT1851.8189894E-1217.013
TTAGACT2250.016.4444454
GGCGTAA1201.9376712E-615.41666713
GCGTAAA1201.9376712E-615.41666714
TCTATAC3250.015.3692313
TCGCTAC855.3673587E-415.23529413
CGAACTA2700.015.07407324
TCGAACG1001.0942004E-414.83
AAAAGTA18150.014.0661152
CGATAGA3950.014.05063237
AAAAAGT18050.014.0415521
GCGAACT2900.014.03448323
ATAGGCG4100.013.98780532
GCCGAGT2151.9826984E-1013.76744212
TAGACTG3100.013.7258075
CGAGTTC2457.2759576E-1213.59183814
Produced by FastQC (version 0.11.5)