FastQCFastQC Report
Thu 28 Apr 2016
ERR1041698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163966
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16080.9806911188905016No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10780.6574533744800751No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG9760.5952453557444836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.5043728577875901No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.41654977251381387No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6750.41167071222082624No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6470.3945940011953698No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6300.3842259980727712No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5950.36288010929095055No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5920.36105046168108024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4720.28786455728626664No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4290.2616396082114585No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3540.2158984179647No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG3130.1908932339631387No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3100.18906358635326836No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3100.18906358635326836No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3060.18662405620677458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3050.18601417367015113No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT2890.176256053084176No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC2780.16954734518131806No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2740.16710781503482428No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2600.15856945952209606No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2480.15125086908261468No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2310.1408828659600161No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC2280.13905321835014575No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.13539392313040507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2190.13356427552053474No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2070.12624568508105338No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2060.12563580254442994No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1840.11221838673871412No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1830.11160850420209066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.10063061854286864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA250.00548277929.632
GTGTAAA250.00548277929.61
ATAACGC250.00548277929.63
CGGCGAT405.909591E-527.7530
CCGGCGA405.909591E-527.7529
CGACCGG405.909591E-527.7526
TATACCA358.8368636E-426.4285725
GATGCGG509.0291705E-625.90000234
TAAACGA451.3164643E-424.66666616
AAACGAT451.3164643E-424.66666617
GCCGACC451.3164643E-424.66666624
AACGATG451.3164643E-424.66666618
GCGATGC451.3164643E-424.66666632
CCGACCG451.3164643E-424.66666625
CGATGCG451.3164643E-424.66666633
ATAAACG451.3164643E-424.66666615
CCGACCA551.8897914E-523.5454549
AGAGTGT400.001924171623.1256
CGACCAT502.688434E-422.210
TGCGGCG502.688434E-422.236
Produced by FastQC (version 0.11.5)