FastQCFastQC Report
Thu 28 Apr 2016
ERR1041697.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041697.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11723276
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1160210.9896636400951406No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA831320.7091191915979799No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG797100.6799293985742552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT559930.4776224666211049No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC535970.4571844934811737No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG483640.4125468000582772No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT455510.38855180070826617No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG450440.38422707099960796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434100.3702889874809738No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG416900.35561732061925355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT325720.27784042617439014No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA323580.2760149978555482No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT253760.21645826644361185No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC245760.20963423534513728No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC235480.20086535538359754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT233220.1989375665982785No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC227260.193853663429915No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG226870.19352099191386435No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT223720.19083402966884No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA200550.17106992959988318No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA195110.1664295884529205No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG194930.16627604775320484No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC188960.1611836145459682No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT174620.14895153880195264No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG170390.14534333235863423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC169680.1447376995986446No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC165950.14155599509898087No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT145060.12373674389308928No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC141830.12098154133708017No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT137060.11691271279461474No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG134030.11432811101606752No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131760.11239179219187537No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA129600.11054930379528725No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG124540.10623310412550212No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118450.10103831045178838No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG117280.10004029590363649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA68450.016.2972972
TATAACG18250.015.3068492
CTAGTAC6100.015.1639353
AAAAAGT72900.015.1502061
AAAGTAC76950.014.6653663
TTAGACT8750.014.5885724
GTATAAC22350.014.4854581
AGTACTC80300.014.4682455
AAGACGG30600.014.14705855
TTAACGG5500.014.12727235
AAGTACT86350.013.9473074
ATAACGC37950.013.7957843
ACGGACC28550.013.5429088
CGAACGA10300.013.29126216
GATACAT16000.013.0656241
GACGGAC29900.012.9933117
TATACTG14700.012.96258455
CGTTATA3150.012.9206342
CGTTAAT4050.012.7901231
GTATTAG31100.012.7299021
Produced by FastQC (version 0.11.5)