FastQCFastQC Report
Thu 28 Apr 2016
ERR1041696.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041696.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5519045
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC827761.4998246979323415No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG690551.2512128457006602No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA603791.094011735726018No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC381930.6920218987161728No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG365480.6622160174450471No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT336900.6104316960633588No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG328390.5950123617401198No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG322250.5838872486091343No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA225690.40892944340914056No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT208930.37856187075843734No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC198930.3604427939978746No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC182040.32983967334928416No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT180990.3279371702894251No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG174920.3169388906957635No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC170560.30903897322815815No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC170440.3088215443070314No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA166280.30128400837463726No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG134210.2431761292035126No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC131450.23817526401759725No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG128140.232177849609851No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC124640.22583617274365403No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT122190.22139699893731615No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT116030.2102356476528095No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC112460.2037671372492886No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG98040.17763942856055712No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA96810.1754107821190079No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG95170.17243925353027562No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG93570.16954020124858557No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT90920.16473864590703644No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG89380.1619483080859098No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT83250.15084131403168483No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77830.14102077442745983No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG74870.13565752770633327No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT74740.13542197970844594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70720.1281381108506997No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG63760.11552723342534806No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT60210.10909496117534827No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT58760.10646769504506669No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT57390.10398538152886957No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT57060.10338745199577101No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC56180.10179297324084148No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG55670.10086890032605278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT32100.021.4392531
AAAAGTA33900.020.5737462
TTAACGG3200.016.76562535
AAAGTAC43150.016.4206263
AAGTACT46400.015.7887934
AGTACTC44850.015.7569675
GATACAT7550.015.682121
TATACTG5150.015.0873785
GATACAA8900.014.9662911
ATTAGAG9150.014.9617493
TAACGGC3600.014.90277836
TAGAGTG6750.014.85
GTACTCC20300.014.76354736
CGAACGT3550.014.5915494
GATACAC7500.014.5533341
GTATTAG13250.014.3811321
TTAGAGT5850.014.230774
CGTATAC1702.442539E-814.1470593
TCTATAC4350.014.0344823
TTAATAC4250.013.9294123
Produced by FastQC (version 0.11.5)