FastQCFastQC Report
Thu 28 Apr 2016
ERR1041695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9746147
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1425571.4627011063961994No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1052231.0796369067694136No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1002391.028498749300621No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC577360.5923982061834282No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG557840.5723697785391499No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT534800.5487296672213132No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG527240.540972755695148No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG516760.5302197883943265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT466020.47815818907718094No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA360790.3701873160747524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319840.3281707119746911No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT317290.32555429340435765No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC296010.3037200239233001No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC284180.2915818938499491No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT269050.2760578103326371No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC259830.26659766161950976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT257810.26452504769320634No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA250420.2569425640717301No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG225130.2309938481330109No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT210050.21552106694060738No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG208160.2135818390590661No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC206610.21199146698690263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC204930.21026770886997703No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG200570.20579414613795585No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC197880.20303408105787857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT183250.18802302078965155No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT175520.1800916813587975No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG165450.16975939312222563No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA155890.1599503885997205No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG145000.14877674223464926No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC143870.14761730969171716No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT141320.14500089112138367No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG139660.14329765393442148No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT139090.14271280743046458No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA136580.14013743072005788No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG130320.13371437964151373No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG125220.12848154250084673No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC118110.12118635189885807No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT118040.1211145286439862No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT113880.11684617521159901No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT113870.11683591474661731No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT107010.10979723576917114No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT105860.10861728229627564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA60750.018.1497922
TATAACG14250.018.0456142
AAAAAGT62700.017.496811
GTATAAC16650.017.111111
TTAACGG4500.016.85555635
GATACAT12300.016.2439021
TAACGCA17350.015.6743534
AAGACGG22000.015.5568195
AAAGTAC73200.015.3661213
TAACGGC4900.015.1020436
GACGGAC21050.015.0285047
AGTACTC76550.014.9595035
ACGGACC21650.014.86836058
AAGTACT81350.014.5998774
CGCAAGA23150.014.4643632
ATAACGC30200.014.3956963
CTAGTAC4600.014.0760873
GTACTCC37700.014.03448336
CGAACGA8450.014.01183516
AGACGGA23800.013.6806726
Produced by FastQC (version 0.11.5)