FastQCFastQC Report
Thu 28 Apr 2016
ERR1041694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6097069
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1562852.563280815749338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1141011.8714073926340673No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1039761.7053439939748098No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC637921.0462732175082816No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG611461.0028753159919956No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT531340.8714679135171343No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG521780.8557882484190353No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG512100.8399117674410441No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC376730.6178870535990326No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA369930.6067341537384603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT345110.5660260692473712No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT327090.5364708846168544No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT301790.49497553660619553No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC289420.4746870996539485No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC287880.47216129586199534No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA247460.4058671469848873No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG241860.3966824059232395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234640.38484065048304356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224070.36750445172918333No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC219320.3597138231501071No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG215500.3534485176401973No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT209690.3439193487887377No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC209280.34324689453243845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC198570.325681077252037No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG197230.32348329992657127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193430.3172507970633103No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT185320.3039493238472453No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG184480.30257161268799815No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG178350.29251760149015865No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG167710.27506659347302775No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA163570.26827644561673814No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT160190.2627327983331007No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC146350.24003336685217108No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT143190.23485054868166982No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT139520.22883126302162562No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC127750.20952690546884087No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT126520.20750954269994323No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG125930.2065418646238053No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG122360.2006865921970048No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT122180.20039136837716617No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT117030.191944686865115No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT112600.18467890063241862No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA108240.1775279236629928No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT98210.16107739636864862No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC93440.15325396514292358No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA86560.14196985469575626No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA83550.1370330563751206No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT83530.13700025372847183No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.13665582593866005No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG80310.13171902761802434No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC79380.1301937045488578No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACA77930.127815512666824No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT77110.12647060415422556No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA76330.12519130093492464No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC76080.12478126785181536No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75940.12455164932527416No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT75830.12437123476870607No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG75720.12419082021213798No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73710.12089415422393941No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT66680.10936402392690651No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA66590.10921641201698717No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT65580.1075598783612257No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG65400.107264654541387No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA64930.10649379234514157No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT63130.10354155414675477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1750.024.3142872
GATACAT8300.020.0602421
AAAAGTA49200.019.7408542
AAAAAGT49000.019.4061221
GATACAA11200.019.1607151
TATAACG10650.018.2394372
TAAGTTC5900.017.872883
GTATAAC11900.017.5672281
CGTATTA1801.0913936E-1116.44444515
CCACTCT14850.016.3198641
GTACTCC33700.016.30415336
GATAAGT6700.016.0149251
CACTCTT16150.015.6934992
AAGTACT66500.015.1338354
AAAGTAC63600.015.1257863
AGTACTC65150.014.9363025
TACTCCT37600.014.90824437
ATACAAC19050.014.8582681
TAACGCA13700.014.8540144
ATAAGTT6850.014.8540142
Produced by FastQC (version 0.11.5)