FastQCFastQC Report
Thu 28 Apr 2016
ERR1041693.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041693.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6901334
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1021461.480090660733128No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA678370.9829548895909109No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG655270.9494830999340127No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC411970.5969425621191498No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG346880.5026274630383054No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG338360.4902820237362805No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT335910.48673198543933677No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG334880.4852395203593972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT311240.45098527328194815No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA242820.3518450201077067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224880.325850045802739No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC218280.3162866773293395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217280.3148376821060972No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT185230.26839738520117995No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC184780.26774533735072087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT184520.26736859859267786No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC181000.26226813540686483No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT177170.25671848370184663No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG160200.232129034763424No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA147360.21352393609699225No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG136990.19849785563196914No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT133260.19309310344927516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC128110.1856307780495771No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG125640.1820517598481685No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC125050.1811968526664555No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC124530.18044337515036948No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT117650.17047428801446213No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT116190.1683587549885283No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG110430.1600125425026524No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA107930.15639005444454654No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG107510.15578147645078472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT105420.15275307643420824No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA102560.14860895009573513No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG96920.14043661703664828No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC93300.13519125432851098No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93140.1349594150927922No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT89920.13029365047395183No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84560.12252703607737286No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT81300.11780331164960281No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80570.1167455451366359No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG79250.114832871441956No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG78990.11445613268391298No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT72980.10574767139222649No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC72620.10522603311185924No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT72390.1048927642105135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1800.023.638892
AAAAGTA41650.017.2785112
AAAAAGT43450.016.4349821
GATACAT9650.015.9119161
TTAGACT5000.015.914
AAAGTAC46700.015.56852153
AGTACTC48100.015.3461545
TAACGGC2850.014.92982436
AAGTACT50850.014.8800394
CGAGCCG17250.014.80000115
TTAACGG2900.014.67241435
TATAACG9550.014.5287972
AAGACGG17700.014.2146895
CGGTCCA12250.013.89387810
CGAACGA11100.013.516
GGTTATC9600.013.4895842
GTACTCC28100.013.43060536
TCTAGCG12300.013.38617928
GTATAAC11900.013.3697471
TATATCG1254.8512524E-513.325
Produced by FastQC (version 0.11.5)