FastQCFastQC Report
Thu 28 Apr 2016
ERR1041692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5493348
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC598911.0902458755571283No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA396820.722364576211083No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG373570.6800406600856163No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC254990.46417958592829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250360.45575121037298205No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG216500.39411302542638843No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT205020.3732150229695989No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG203160.3698291096795615No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG193630.35248085502684334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT184020.3349869696949838No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173540.3159093507274616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158890.2892407326096945No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA150520.27400412280452646No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC125840.22907705828940747No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT109300.198967915376925No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC108860.19816694664164733No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC107590.19585505961027772No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT106850.19450797582821988No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG102070.1858065427495218No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA94140.17137090167963145No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA89890.16363427185024504No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG79210.14419257618486941No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT78650.14317316143087966No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG78470.14284549240281155No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC76770.139750840471057No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC74380.13540012393170794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC74320.1352909009223519No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT72320.13165013394381714No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT67200.12232977047876815No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63970.11644993180843449No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG63910.11634070879907846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT63300.11523027487062534No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62860.1144293061353477No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG61270.11153489638741257No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA61160.11133465420359315No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC60140.10947786304454042No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG57800.10521816567965474No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56560.10296089015296317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT500.00703818618.49999822
CGTTATA1005.8859314E-616.652
TATAACG8800.015.9772732
TAACGCA10650.014.9389674
AAAAGTA29900.014.9113722
AGTACTC32000.014.8578125
AAAGTAC30550.014.7757783
GTATAAC11600.014.6724141
TAAGTTC5600.014.5357153
CTAACGC1152.2127406E-514.4782613
AAGTACT34250.014.1518254
AAGACGG11050.014.0633495
AAAAAGT31850.013.8241751
TATACTG5650.013.7522135
ATAACGC19800.013.3611123
TACCGTC10250.013.3560987
GGGGTTA6700.013.2537326
ACCGTCG10050.013.0696538
ACGGACC11850.012.8016888
GTACTAG2753.6379788E-1212.7818181
Produced by FastQC (version 0.11.5)