FastQCFastQC Report
Thu 28 Apr 2016
ERR1041691.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041691.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064393
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC724321.1943816965688074No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA472040.778379633377982No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG450870.7434709458968112No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC308640.5089379926399888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT279680.46118383158875087No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG261580.4313374809317272No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT246420.4063391010444079No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG244860.40376670839109535No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG230100.3794279163635998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT198340.32705664029359577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197810.32618268637932935No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA184920.3049274675965097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165890.27354757516539574No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC152380.2512699951998494No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC134140.22119278879188733No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC132920.21918104581942496No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132700.21881827249652192No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG129170.2129974096335775No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT128140.2112989708945314No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA104420.1721854108069843No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT98590.16257191775005347No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG96840.15968622086332465No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG96590.15927397845093483No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA94400.1556627349183999No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC93170.15363450224944195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC92760.15295842469312262No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC88890.14657691214932805No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT83120.13706235727137078No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT81710.13473731006549214No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG78930.13015317443971722No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG77920.1284877150936623No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT76030.12537116245599517No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74420.12271632132020469No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC73720.12156204256551315No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA72780.12001201109492739No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68460.11288846220883111No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG67330.11102512650482908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65500.10800751204613553No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT64000.10553405757179656No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG62080.10236803584464266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG3100.017.30645235
TAACGGC3150.016.44444536
TTAGCGA2250.015.62222227
TATACTG6700.015.1865675
AAAAGTA35500.014.9042242
GTATAAC10600.014.66037751
TAATACT12300.014.5894314
TATAACG7950.014.4276742
AGTACTC37800.014.3888875
AAAAAGT37100.014.1617251
AAAGTAC37900.014.0092343
CACGCAG9150.013.950825
CGTTATA1753.574496E-813.7428572
TATCACG8800.013.6647732
GTATCAC10300.013.6504861
TAGAGTG9200.013.4728265
TAGACTA5100.013.4215695
AAGTACT42450.013.379274
AAGACGG15000.013.325
TATTAGA10350.013.2270532
Produced by FastQC (version 0.11.5)