FastQCFastQC Report
Thu 28 Apr 2016
ERR1041690.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041690.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6091431
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC554380.91009813621791No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA346360.5686020247130764No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG330070.5418595400653804No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC236720.3886114773359495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197390.3240453679931694No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG194160.3187428372741971No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT187230.30736620015887894No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG186200.30567530027016643No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG165700.272021467533655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143930.23628273881785739No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA137540.2257925929063302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136970.22485685219121748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119660.19643988415858274No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC112860.18527666159232534No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC103150.16933623642786072No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC102660.16853182774293923No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97170.15951916717106376No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT96320.15812376435028155No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG94750.155546373257778No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA83690.13738972008383582No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG75200.12345210837978794No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT75160.1233864423646923No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73750.12107171533257129No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA69770.1145379468305559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC69740.11448869731923417No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC68230.11200980524937408No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT67170.11026965584933983No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC65000.1067072745304018No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT62400.10243898354918572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7500.018.9933342
GTATAAC11100.017.6666661
TAACGCA8650.017.3236984
TTAACGG2650.014.660377535
ATAACGC18450.014.5392953
GTACTAG3500.014.2714291
CGAACGA9100.013.82417716
TACGTCG1356.577602E-613.7037045
AAGACGG15700.013.4331215
TAACGGC3050.013.34426136
TAATACT12650.013.3083014
GTATTAG18050.013.1191141
GACGGAC15100.013.109277
GTTAGAC3250.013.0923073
CGAGCCG15150.012.82178215
ACGGACC15650.012.64856158
CGCAAGA16350.012.5596322
AAAGTAC31400.012.5493633
TATACTG7550.012.4966885
TATCACG6550.012.4274812
Produced by FastQC (version 0.11.5)