FastQCFastQC Report
Thu 28 Apr 2016
ERR1041688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7401207
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC443350.5990239159639772No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA286320.3868558195980737No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG244820.3307838843042763No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC197980.26749690962568673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182890.24710834327427944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG145060.19599505864381309No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144510.19525193660979892No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT143630.19406294135537625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT141720.19148228120089061No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG138500.1871316394744803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123570.1669592540784226No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG119010.16079809685095958No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA105140.14205791028409284No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC83380.11265730035654996No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC78310.10580706633391013No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC76460.1033074740376806No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG76370.10318587225029646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG5700.015.2543872
TTAACGG5650.014.4070835
TAACGGC5850.014.23076836
GTATAAC9250.014.01
TAATACT17500.013.6371434
GTATTAG22850.013.601751
CGTTATA2355.638867E-1113.3829792
CGAGCCG19400.012.77835115
ATTAGAG18100.012.7762433
AGTACTC28450.012.5500885
CACTCTA16850.012.40652759
AATACTG16950.012.3333345
CTACGCT1653.8108537E-612.3333334
ACTCTAA17000.012.18823410
TAGGGGT9600.012.1406264
TAACGCA8250.012.1090924
CGAACGA14200.011.98591516
AGTACCG6800.011.9705895
ATAACGC20650.011.9152553
AAGTACT31150.011.878014
Produced by FastQC (version 0.11.5)