FastQCFastQC Report
Thu 28 Apr 2016
ERR1041687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541813
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC199851.2962012902991478No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG181651.1781584407447596No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA129570.8403742866352794No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG101280.6568890001576067No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT79740.5171833419487318No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79140.5132918194359497No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG72150.4679555821620391No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC68450.44395785999988324No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57900.3755319224834659No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC57890.37546706377491956No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA50940.33039026133519434No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC46140.2992580812329381No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT44440.2882321007800557No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC44330.2875186549860456No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA38220.24788998406421534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC31740.20586154092616937No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30710.19918109394589356No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT30350.19684618043822436No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG29370.19049002700068038No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC28710.18620935223662013No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG28510.1849121780656928No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG26080.16915151188892558No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT25560.16577885904451448No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG25050.16247106490864976No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA23710.15377999796343655No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG23690.15365028054634383No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC22280.14450520264130604No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT19680.1276419384192506No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC19480.12634476424832325No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG18980.12310182882100489No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG18770.12173979594153118No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT18490.1199237521022329No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT16240.10533054267930028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATT502.7026818E-422.214
TAACGGC603.7273483E-521.58333436
TTAACGG555.144188E-420.18181835
AAAAGTA6550.019.2061082
CTAGACT1306.9667294E-1018.54
GTTATGC1002.8765135E-718.53
AAAAAGT6850.018.3649641
GACGGAC3250.016.5076927
AAGACGG3750.016.285
GGGTTAT803.383502E-416.18751
CGTAGTC700.002593031615.85714328
TAGAGTG2003.6379788E-1215.7255
ACGGACC3500.015.3285728
GATATAT855.365625E-415.2352941
CGTATTA750.004105250414.815
ATATAAG1001.09374814E-414.81
TATTAGA1752.239176E-914.82
TATAGGA750.004105250414.82
CCTATAT750.004105250414.81
TAAACAC1405.998154E-714.5357155
Produced by FastQC (version 0.11.5)