FastQCFastQC Report
Thu 28 Apr 2016
ERR1041686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10153622
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1319341.299378684768844No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG913770.8999448669647147No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA844040.8312698660635585No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC515040.5072475615105624No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG461610.45462594530306527No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG446420.4396657665609376No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG443190.4364846357290039No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT441410.4347315667256472No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA315250.3104803389371793No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC297830.29332389958972277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT294120.2896700310490188No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT260220.25628293036711436No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT251020.24722212428234966No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC248570.24480919222716782No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC244020.24032803269611572No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG229790.2263133293715287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208620.20546362667430398No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC207720.20457724347035963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205270.20216431141517774No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG186810.18398360703205222No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA185800.1829888881031813No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT180100.17737512781153367No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG179580.17686299529369914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT171840.16924009973977758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC167690.16515288829936745No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC165130.16263162051925903No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG150680.14840024574481894No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT149310.1470509735343703No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT148440.14619413643722406No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG144420.14223495812627257No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT141060.1389257941648803No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG135790.1337355280706727No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA132260.1302589361707576No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT130480.12850586716740095No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC129470.12751114823853005No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT119250.11744577452262848No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG114650.11291537148024618No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG103160.10159921257655642No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC102120.10057494754088737No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT101940.1003976709000985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG11200.016.5178592
AAAAGTA56300.016.495562
GTATAAC14100.015.8758871
AAAAAGT58350.015.7892031
AAAGTAC62500.015.00723
TAACGCA13950.014.5878134
AGTACTC66150.014.4588065
AAGACGG37650.014.39707855
AAGTACT68450.014.3783784
GTACTCC36800.014.32744636
CGTTATA2700.013.7037042
TATACTG10450.013.6315785
CGCAAGA41000.013.175612
CAAGACG43900.013.0637824
TTAACGG3850.012.97402635
GGTTATC17700.012.9604522
CGAGCCG33100.012.85498515
TGTACCT56450.012.84676735
TATACCG2451.1459633E-1012.8367345
GACGGAC41250.012.7818187
Produced by FastQC (version 0.11.5)