FastQCFastQC Report
Thu 28 Apr 2016
ERR1041685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946204
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC434350.36358829968080236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT310830.26019143821752916No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA276530.23147938876650695No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG245980.20590641177733113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT232640.19473968467305597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209330.1752272102502184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207660.17382927664720943No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG189260.15842689443441615No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC184340.15430843136447359No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG172920.14474890936066387No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT161020.13478758608173774No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT142650.11941031644863925No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA132290.11073810559404478No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG125040.1046692321678083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG10300.019.5776712
TAACGCA13350.015.7977524
AAGACGG39550.015.529715
GTATAAC15450.015.2071211
TATACTG15700.014.1401275
TAATACT27250.013.9853214
ATAACGC47300.013.4154333
TCGTTTA28950.013.35578630
GACGGAC44150.013.2831267
ACGGACC45000.013.2377788
GTATTAG44500.013.1786531
CAAGACG50550.013.1750754
ATTAGAG29650.013.1652613
TTAACGG8800.013.03409135
CGCAAGA46150.013.0281692
AGACGGA46750.012.9005366
TAGGGGT17850.012.85154154
CGAACGT12100.012.8429764
TACCGTC32700.012.7293587
CGGACCA47650.012.6956989
Produced by FastQC (version 0.11.5)