FastQCFastQC Report
Thu 28 Apr 2016
ERR1041684.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041684.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5718927
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1123041.9637250134509499No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG755151.3204400056164383No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA753011.3166980449304564No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC450610.7879275255655476No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG410930.7185438806965013No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT408760.7147494626177253No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG408410.7141374597017938No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG399380.6983477844707583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT297340.5199227057802975No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA284510.49748842746200467No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC253770.4437370856456115No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT232150.40593279123863624No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC224590.3927135282545135No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC215510.3768364240354878No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT213130.3726748042071528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190800.3336290181707163No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG189940.33212523957728435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188610.32979962849674427No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA170890.29881479515300685No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG164070.2868894811911395No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT157900.2761007440731452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT156880.2743171927181445No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC155820.2724636981727516No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG154810.270697632615349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC150480.26312628225539514No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC142540.24924255896254666No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT130370.22796234328572476No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG126710.22156254136483997No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA118700.20755641748880518No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG118500.20720670153684426No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG117790.20596520990738298No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC114290.19984518074806693No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT101650.17774313258413685No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG101230.1770087290850189No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT100220.1752426635276163No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT94910.1659577050030539No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG88450.15466187975471624No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC87390.15280838520932336No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT86190.15071008949755785No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT85840.15009808658162624No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT84750.14819213464343925No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT83000.14513212006378118No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA80210.1402535825339264No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT69340.12124652054485045No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA67340.11774936102524128No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67320.1177143894300452No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG66180.11572100850386795No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT64860.11341288322092588No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT61830.10811468654871796No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61250.1071005102880313No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61060.10676828013366842No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC60950.10657593636008993No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60600.10596393344415832No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG58870.10293889045969637No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA58530.10234437334136282No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC57580.10068322256954845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT42500.019.6752951
AAAAGTA42950.019.5552962
TATAACG8550.019.0409362
GATACAT6900.018.51
GTATAAC10850.018.4147471
GATAAGT4900.015.8571431
AAAGTAC54100.015.4907583
TGCGACG1201.9386807E-615.41666622
TAACGCA11500.015.2826094
CACGCAG6700.015.1865675
ATAAGTT4800.015.0312492
AGTACTC58000.014.9275875
ATAACGC18700.014.8395733
AAGTACT58950.014.8125534
GACGGAC13500.014.87
AAGACGG14050.014.7473315
TAAGTTC5100.014.5098043
TGTACCT33350.014.31184435
TATCACG6500.014.2307692
ACGGACC13850.014.1588458
Produced by FastQC (version 0.11.5)