FastQCFastQC Report
Thu 28 Apr 2016
ERR1041683.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041683.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91227
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4590.5031405176099182No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG3130.34310017867517295No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2820.3091190108191654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.2619838425027678No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1880.20607934054611027No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1720.18854067326559024No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1600.1753866728052002No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1600.1753866728052002No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1480.1622326723448102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1480.1622326723448102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1400.15346333870455017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.15017483858945269No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1100.12057833755357515No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1010.11071283720828264No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT940.10303967027305513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCT250.005470176729.69
ACAGGGG358.806676E-426.428573
CAGGAGT555.0933706E-420.1818184
CCAGAGC701.203655E-418.58
CCACGGA500.006986542618.526
CCAGGAG700.002568039615.8571423
CTGCCAG750.00406600914.823
TCTCTTA850.00931819913.05882537
CTGTCTC1306.861653E-512.80769337
ACGCAAA1050.002611528412.33333315
TCTGCGT2901.0913936E-1112.1206910
TGCGTTG2901.0913936E-1112.1206912
CTGCGTT2951.4551915E-1111.91525511
GCTTCCC2851.05501385E-1011.6842127
CGTTGAT2951.9463187E-1011.28813614
TGCTTCC3002.6011548E-1011.126
GCGTTGA3002.6011548E-1011.113
TACCACT3259.094947E-1110.81538520
CTCTGCG3259.094947E-1110.8153859
TTGATAC3104.620233E-1010.74193516
Produced by FastQC (version 0.11.5)