FastQCFastQC Report
Thu 28 Apr 2016
ERR1041682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7716876
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC421060.5456353063078894No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA282720.3663658713707464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG262370.3399950964613141No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC167120.21656431955107222No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT152580.19772249806787098No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG151490.19631000938721838No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG147040.19054342715886582No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG141910.1838956593315741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130650.16930426250207986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA102340.1326184326403586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94170.1220312468413384No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT91620.11872680084531616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90080.11673117463595371No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC83430.10811369782279773No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC82680.10714180194161471No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC80350.10412244540407282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG10950.015.2054795
TATAACG5300.014.66037752
CGTTATA1904.5656634E-1014.6052642
TAGAGTG13850.014.55956655
AAGACGG28900.013.8910035
ATTAGAG17450.013.8882523
TTAGAGT15050.013.6445194
CGCAAGA28700.013.6010462
GTATAAC8200.013.5365861
CGAACGA15750.013.507936516
TCGTTTA16050.013.25545230
ATAACGC23500.013.1468093
TATACCG3450.012.8695655
ACGGACC30050.012.8668898
GACGGAC29750.012.8100837
ACGAACG16350.012.78593315
CTAGTAC3500.012.6857143
CGAACGT7750.012.6516134
TCTATAC7650.012.5751633
TATATCG2805.456968E-1212.5535715
Produced by FastQC (version 0.11.5)