FastQCFastQC Report
Thu 28 Apr 2016
ERR1041681.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041681.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5348970
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC872011.6302390927599146No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA588931.1010157095665147No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG549401.0271136312224598No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC367560.6871603318021975No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT312160.5835889900298562No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG306660.5733066366048043No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG300750.5622577804698848No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG290200.5425343570818306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT271090.5068078527267866No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA214150.4003574519954309No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186240.34817918216030375No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC185890.3475248505787096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178320.333372593228229No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166480.311237490582299No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC165250.3089379824526965No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC159590.2983565060189158No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT158450.2962252545817232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150780.28188604535078715No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG146070.2730806117813336No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA133120.2488703432623477No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120310.22492180737599948No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT118440.22142580721148183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC116360.21753720809800764No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG114870.21475162507922085No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC109180.2041140630813035No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC106840.19973938907864502No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT104740.19581339958907978No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT93760.17528608311506702No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT91430.1709301043004541No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90370.16894841436762592No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG89320.16698541962284327No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA89150.16666760142606893No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA88270.16502242487806065No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC86220.1611899113287231No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG82840.15487093776932753No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT77310.14453249878013896No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73180.13681138611732727No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG73080.1366244342368718No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT71770.13417536460290486No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG67990.12710858352168736No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT67500.12619251930745545No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT65850.12310781327993987No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC63820.11931269010669344No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63760.11920051897842014No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61210.11443324602680516No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT59410.11106811217860635No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT57080.10671213336399343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA2650.017.4528312
AAAAGTA36700.017.3910082
CACGCAG6950.017.035975
AAAAAGT38600.016.4391171
AAAGTAC40650.015.974173
TATAACG9700.015.4484542
CTAGTAC1802.0190782E-1015.4166663
GTATAAC10050.015.2786061
AGTACTC43750.015.1805715
TATCACG7850.015.0828032
CGTTAAT2109.094947E-1214.97619151
GTTATAC4600.014.8804353
AAGTACT45850.014.8484184
CGAACGT2900.014.6724144
GATACAT8600.014.6279071
GATACAC6600.014.5757591
TTATACC8950.014.46927454
TAATACT11150.014.4349784
ATACCGA1803.3396645E-914.3888886
AGTACCG5150.014.3689325
Produced by FastQC (version 0.11.5)